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宿主来源
Rabbit抗原名称
Phospho-p53 (Ser15)分子别名
Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53; P53; TP53细胞定位
NucleusAccession
P04637克隆号
S-4249抗体类型
Recombinant mAb抗体同种型
IgG翻译后修饰类型
磷酸化反应种属 ?
Ms, Rt阳性样本
Etoposide treated L-929纯化方式
Protein A浓度
0.5 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied
应用
WB
稀释度
应用 稀释度 推荐种属 WB 1:1000 Ms
Phospho-p53 (Ser15) is a critical biomarker of p53 activation, playing a key role in cellular responses to DNA damage and stress. When cells experience DNA damage, p53 is phosphorylated at serine 15, which stabilizes the protein and enhances its transcriptional activity. This phosphorylation event triggers a cascade of downstream effects, including cell cycle arrest, DNA repair, and apoptosis, depending on the severity of the damage. In senescent cells, phospho-p53 (Ser15) is often localized in the nucleus, where it interacts with other proteins such as FOXO4 to maintain the senescent state and prevent apoptosis. This regulatory mechanism is crucial for understanding the balance between cellular senescence and apoptosis, and it has significant implications for aging, cancer, and other age-related diseases.
免疫印迹
WB result of Phospho-p53 (Ser15) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-p53 (Ser15) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated L-929 whole cell lysate 20 µg
Lane 2: L-929 treated with 30 µM Etoposide for 2 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 53 kDa
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